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CLS Cell Lines Service GmbH
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BPS Bioscience
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Alcami Inc
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National Centre for Cell Science
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Korean Cell Line Bank
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ATCC
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Image Search Results
Journal: ACS nano
Article Title: Re-Engineered Pseudoviruses for Precise and Robust 3D Mapping of Viral Infection.
doi: 10.1021/acsnano.3c07767
Figure Lengend Snippet: Figure 1. Development of clickVSVs bearing the SARS-CoV-2 spike (S) Glycoprotein. (a) Schematic representation of the SARS- CoV-2 virus, the vesicular stomatitis virus (VSV), and the clickable pseudovirus (VSVΔG-eGFP-SARS-CoV-2-Sclick), generated using Genetic Code Expansion via amber suppression using orthogonal tRNA/tRNA-synthetase pairs (aaRS/tRNAaa). (b) Widefield fluorescence images of GFP (green) and Hoechst (white) of infected Vero E6 cells after 24 h of infection with VSVΔG-eGFP- SARS-CoV-2-S variants. (c) Influence of click-labeling on infectivity of clickVSVs: without (gray) or with prior click-labeling with Tetrazine-Cy5 (Tet-Cy5) (magenta). (d) Neutralization of wildtype VSVΔG-eGFP-SARS-CoV-2-S and clickable VSVΔG- eGFP-SARS-CoV-2-SCy5 with a neutralizing antibody (+nAB) or additional treatment with soluble ACE2 ectodomain (+sACE2).
Article Snippet:
Techniques: Virus, Generated, Fluorescence, Infection, Labeling, Neutralization
Journal: Journal of Clinical Medicine
Article Title: Early Cellular and Humoral Responses Developed in Oncohematological Patients after Vaccination with One Dose against COVID-19
doi: 10.3390/jcm11102803
Figure Lengend Snippet: DCC and viral neutralization activity against SARS-CoV-2 infected cells of PBMCs of individuals with OHD before and after receiving one-dose vaccine. ( A ) The capacity of PBMCs from individuals with OHD to eliminate SARS-CoV-2-infected Vero E6 cells was determined by quantifying the production of Renilla (RLUs) after co-culture for 1 h. ( B ) DCC was assessed by measuring the activity of caspasa-3 in SARS-CoV-2-infected Vero E6 cells co-cultured with PBMCs from individuals with OHD, in comparison with healthy donors. Each dot in the graphs corresponds to mean ± SEM. Statistical significance between groups was calculated using one-way ANOVA test.
Article Snippet:
Techniques: Neutralization, Activity Assay, Infection, Co-Culture Assay, Cell Culture, Comparison